Rewiring the System


Helter Skelter

Posted in Uncategorized by rewiringangel on January 12, 2011
Tags: , , , , , , , , , , ,

The Ride:  The Slide from the individual cell out to the Universal Ride we are engaged in on this Big Blue Ball.

Listen to this while you read on…

 

I am talking about balance from the inside out. Bam you are pushed backward from your center. You are Helter Skelter with the sticky stuff not holding us together. Yes, balance and unbalance: spin is all and be all, all the inflammation we all carry around makes even our cells Helter Skelter.

Oh we live in times where the rhythm is osculating out of harmony. I can see the number of stressors reorganizing the structure of life. From the inside of even the single cell where creative separation from one to two so to in the complexity of society more and more people lose their natural health. The merry-go-round pushes our thoughts and actions back on ourselves.  I have been thinking about how science can lead with more grace than philosophy in the our human future. I am in the intersection as are we all of thoughts and feelings. If everything we are and all our emotions and thoughts are gradations of finer and finer bits of material. It is all the same stuff… We are all moving through places we have never been before and this is the truth.

Cancer is a cellular matrix out of balance. It shows up as being tripped up by the smallest change.  Road rage is such a useless expansion of energy. There is you in your metal skin with and air pocket separating you from the next medal envelope that is transporting the object of your rage. This is an instance where paying attention to what is real and rhythmic is vital.

I attended a talk on the motion of cells which noted that the spin of the cell as it replicated generates it own internal force. This force is governed by both the internal atmospheres and the external chemicals. The talk is as yet unpublished and as a work in progress I am not comfortable in naming the specifics but I do know that several of the same proteins are part of an article which came to me in an email.

January 11, 2011

STUDY SHOWS HOW STRESS CAUSES EXTENSIVE GENETIC REWIRING

A team of scientists in the United States, South Korea, and Switzerland has uncovered a vast, complex network of 160,000 genetic interactions within yeast cells that changes dramatically when the cells are subjected to stress.

The “rewiring” of this genetic network is much more extensive than scientists previously thought. About 70 percent of the genetic interactions that took place when the cells were under stress did not take place in normal, unperturbed cells.

“The stress response is dynamic,” says Nevan Krogan, PhD, an associate professor of Cellular and Molecular Pharmacology at UCSF and an affiliate of the California Institute for Quantitative Biosciences (QB3). “In the cell, things are constantly changing and functional modules are being rewired.”

Krogan and Trey Ideker, PhD, chief of the Genetics Division at the UC San Diego School of Medicine, led the study, which was described in the journal Science last month, with first author Sourav Bandyopadhyay, PhD – a fellow at QB3 and the UCSF Helen Diller Family Comprehensive Cancer Center – and colleagues at Albert Einstein College of Medicine, Seoul National University in Korea, and ETH Zurich in Switzerland.

The new work has implicated several genes that were never before known to be involved in stress response, with immediate implications for scientists who study biological responses to stress, the authors say. The technique used also may prove useful for studying a wide variety of human diseases, by providing a new way of examining broadly how cells deal with stresses, diseases, drugs, or other challenges.

How a cell is wired genetically – the exact way its thousands of genes interact and “talk” to one another – is a critical issue for understanding the inner workings of the cell. In the last decade or so, the revolution in DNA sequencing has led to a wealth of new information about which genes are present and active in many types of cells.

Often this data is static, however, and is limited to information about which genes are present but not how these genes interact or how these interactions change over time. The difference is analogous to comparing a photograph with a video.

Imagine a busy playground full of children in the summer. A psychologist examining child behavior might find a photo of the playground useful. It would reveal the structures, the people, and perhaps many of the human interactions. But a video might reveal rich details not seen in the static image, such as which kids are playing with each other, which are playing by themselves, which adults seated on the benches are attentive, and which are distracted. A video might even reveal how a dramatic change to the environment, like the sound of an approaching ice cream truck, alters the children’s play. The same is true for the landscape of a living cell, says Krogan.

“What we’d really like to have,” he says, “is an animated movie.”

Up to now, however, there hasn’t been a way to grab more than a single snapshot with respect to large-scale interaction networks.

Now, using a new technique called differential epistasis mapping, Krogan and his colleagues have been able to take a step toward a more animated view of the dynamic rewiring inside cells. Using the technique, they have generated two snapshots of about 160,000 genetic interactions within yeast—one set of interactions that exists under normal conditions and one set that exists when the cells are stressed, having been exposed to a DNA-damaging chemical called methyl methanesulfonate.

The snapshots reveal how widely yeast cells rewire their genetic networks to deal with the DNA-damaging stress.  In their article, Krogan and his colleagues detail how the technique helped them to identify sets of genes involved in the cell’s response to the DNA damage that were not previously known to be involved in DNA repair.

“Nobody could have predicted the connections we are finding in the presence of stress,” says Krogan.

The article, titled, “Rewiring of Genetic Networks in Response to DNA Damage,” appeared in the Dec. 2, 2010, issue of the journal Science and online at

http://www.sciencemag.org/content/330/6009/1385.full.html.

Bandyopadhyay was affiliated with UC San Diego, La Jolla, CA, at the time of the research, as are co-authors Ryan Chuang, Wilbert Copeland, Janusz Dutkowski, Eric J. Jaehnig, Dwight Kuo, Richard D. Kolodner, Katherine Licon, and Ideker. Additional co-authors include Monika Mehta and Michael-Christopher Keogh, from Albert Einstein College of Medicine, Bronx, NY; Min-Kyung Sung and Won-Ki Huh, from Seoul National University, Seoul, Korea; Bernd Bodenmiller and Ruedi Aebersold, of the Institute of Molecular Systems Biology and University of Zürich, Switzerland; Michael Shales, Dorothea Fiedler, Kevan M. Shokat and Krogan, of UCSF; and Aude Guénolé, of Leiden University Medical Center, Netherlands. Fiedler and Shokat also are affiliated with the Howard Hughes Medical Institute, San Francisco. Full affiliations can be found in the published article.

The research was supported by grants from the National Institutes of Health, as well as the Frontier Functional Proteomics Project, the Swiss National Science Foundation, Keck Young Investigator fellowship, Searle fellowship and David and Lucille Packard fellowship. Additionally, Kolodner is a paid consultant to On-Q-ity. The authors declare no conflicts of interest in this work.

UCSF is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. For more information, visit www.ucsf.edu.

Helter skelter (ride)

From Wikipedia, the free encyclopedia
This article is about the fairground ride.

A helter skelter in BrightonEngland

helter skelter is afunfair or amusement park ride with a slide built in a spiral around a high tower. Users climb up inside the tower and slide down the outside, usually on a mat. Typically the ride will be of wooden construction and, in the case of fairground versions, designed to be disassembled to facilitate transportation between sites. The term is primarily (but not exclusively) found inBritish English. The ride inspired the Beatles song of the same name and is also mentioned in the songs “Tonight, Tonight, Tonight” from Genesis, “Fade In-Out” byOasis, “Fat Gal” by Merle Travis, “Wild Child” by Enya, “American Pie” by Don McLean and in “Bloodbath in Paradise” by Ozzy Osbourne

 

 

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One Response to 'Helter Skelter'

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  1. Diane Nichols said,

    Very interesting, Ilsa! Thank you.


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